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Abstract
Autochthonous lactic acid bacteria may provide a means of promoting the quality and safety of traditional fermented food products, in particular, artisanal cheeses. Pico cheese is an artisanal, dairy specialty of the Azores in risk of disappearing. Efforts to maintain its quality to the requirements of the modern markets are, thus, necessary. Lactic acid bacteria were isolated from artisanal Pico cheese, identified by sequencing of the 16S rRNA gene, and their potential as starter cultures was evaluated by studying their acidification ability, enzymatic activities (caseinolysis, lipolysis and API-ZYM profile), diacetyl and expolysaccharide production, autolysis, antimicrobial activity against Listeria monocytogenes ATCC 7466, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29523, Pseudomonas aeruginosa ATCC 27853 and Clostridium perfringens ATCC 8357, sensory evaluation of odour formation in milk, syneresis and firmness of the curd. Several of the studied lactic acid bacteria isolates showed interesting properties for practical application as starters in artisanal cheese production. The isolates with the highest number of positive traits and, therefore, the most promising for starter development were Lactococcus lactis ssp. lactis L1C21M1, Lactobacillus paracasei L1B1E3, Leuconostoc pseudomesenteroides L1C1E6, Lactobacillus casei L1A1E5 and L1C1E8.
References
| Akuzawa, R, Fox, PF (2004) Acid phosphatase in cheese. Animal Science Journal 75: 385–391. Google Scholar | Crossref | |
| APQP (1996) Caderno de Especificações do Queijo do Pico DOP, Açores: Associação de Produtores de Queijo do Pico. Google Scholar | |
| Beresford, TP, Fitzsimons, NA, Brennan, NL, Cogan, TM (2001) Recent advances in cheese microbiology. International Dairy Journal 11: 259–274. Google Scholar | Crossref | ISI | |
| Câmara, SPA, Dapkevicius, A, Rosa, HJD, Silva, CCG, Malcata, FX, Dapkevicius, MLNE (2017) Physicochemical, biochemical, microbiological and safety aspects of Pico cheese: Assessment throughout maturation and on the final product. International Journal Dairy Technology 70: 542–555. Google Scholar | Crossref | |
| Cheng, AYY, Fantus, IG (2005) Oral antihyperglycemic therapy for type 2 diabetes mellitus. Canadian Medical Association Journal 172: 213–226. Google Scholar | Crossref | Medline | ISI | |
| Domingos-Lopes, M, Stanton, C, Ross, PR, Dapkevicius, MLE, Silva, CCG (2017) Genetic diversity, safety and technological characterization of lactic acid bacteria isolated from artisanal Pico cheese. Food Microbiology 63: 178–190. Google Scholar | Crossref | Medline | |
| EFSA (2016) Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 4: Suitability of taxonomic units notified to EFSA until March 2016. EFSA Journal 14: 4522. Google Scholar | |
| Franciosi, E, Settanni, L, Cavazza, A, Poznanski, E (2009) Biodiversity and technological potential of wild lactic acid bacteria from raw cows’ milk. International Dairy Journal 19: 3–11. Google Scholar | Crossref | |
| Garofalo, C, Osimani, A, Milanović, V, Aquilanti, L, de Filippis, F, Stellato, G(2015) Bacteria and yeast microbiota in milk kefir grains from different Italian regions. Food Microbiology 49: 123–133. Google Scholar | Crossref | Medline | |
| Gobbetti, M, de Angelis, M, di Cagno, R, Mancini, L, Fox, PF (2015) Pros and cons for using non-starter lactic acid bacteria (NSLAB) as secondary/adjunct starters for cheese ripening. Trends in Food Science and Technology 45: 167–178. Google Scholar | Crossref | |
| González, L, Cuadrillero, AF, Castro, JM, Bernardo, A, Tornadijo, ME (2015) Selection of lactic acid bacteria isolated from San Simón da Costa cheese (PDO) in order to develop an autochthonous starter culture. Advances in Microbiology 5: 748–759. Google Scholar | Crossref | |
| González, L, Sacristán, N, Arenas, R, Fresno, JM, Tornadijo, ME (2010) Enzymatic activity of lactic acid bacteria (with antimicrobial properties) isolated from a traditional Spanish cheese. Food Microbiology 27: 592–597. Google Scholar | Crossref | Medline | |
| González, L, Sandoval, H, Sacristán, N, Castro, JM, Fresno, JM, Tornadijo, ME (2007) Identification of lactic acid bacteria isolated from Genestoso cheese throughout ripening and study of their antimicrobial activity. Food Control 18: 716–722. Google Scholar | Crossref | |
| Hantsis-Zacharov, E, Halpern, M (2007) Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits. Applied and Environmental Microbiology 73: 7162–7168. Google Scholar | Crossref | Medline | |
| Herreros, MA, Fresno, JM, González Prieto, MJ, Tornadijo, ME (2003) Technological characterization of lactic acid bacteria isolated from Armada cheese (a Spanish goats’ milk cheese). International Dairy Journal 13: 469–479. Google Scholar | Crossref | ISI | |
| Holland, R, Liu, S-Q, Crow, VL, Delabre, M-L, Lubbers, M, Bennett, M(2005) Esterases of lactic acid bacteria and cheese flavour: Milk fat hydrolysis, alcoholysis and esterification. International Dairy Journal 15: 711–718. Google Scholar | Crossref | |
| INE (2014) Estatísticas Agrícolas 2014, Lisboa: Author. Google Scholar | |
| Leroy, F, de Vuyst, L (2004) Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends in Food Science & Technology 15: 67–78. Google Scholar | Crossref | ISI | |
| McSweeney, PLH, Sousa, MJ (2000) Biochemical pathways for the production of flavour compounds in cheeses during ripening: A review. Lait 80: 293–324. Google Scholar | Crossref | |
| Mayo, B, Aleksandrzak-Piekarczyk, T, Fernández, M, Kowalczyk, M, Álvarez-Martín, P, Bardowski, J (2010) Updates in the metabolism of lactic acid bacteria. In: Mozzi, F, Raya, RR, Vignolo, GM (eds) Biotechnology of Lactic Acid Bacteria: Novel Applications, USA: Blackwell Publishing, pp. 3–33. Google Scholar | Crossref | |
| Menéndez, S, Godínez, R, Centeno, JA, Rodríguez-Otero, JL (2001) Microbiological, chemical and biochemical characteristics of ‘Tetilla’ raw cows-milk cheese. Food Microbiology 18: 151–158. Google Scholar | Crossref | |
| Monteagudo-Mera, A, Caro, I, Rodríguez-Aparicio, LB, Rúa, J, Ferrero, MA, García-Armesto, MR (2011) Characterization of certain bacterial strains for potential use as starter or probiotic cultures in dairy products. Journal of Food Protection 74: 1379–1386. Google Scholar | Crossref | Medline | |
| Montel, MC, Buchin, S, Mallet, A, Delbes-Paus, C, Vuitton, DA, Desmasures, N(2014) Traditional cheeses: Rich and diverse microbiota with associated benefits. International Journal of Food Microbiology 177: 136–154. Google Scholar | Crossref | Medline | |
| Mora, D, Musacchio, F, Fortina, MG, Senini, L, Manachini, PL (2003) Autolytic activity and pediocin induced lysis in Pediococcus acidilactici and Pediococcus pentosaceus strains. Journal of Applied Microbiology 94: 561–570. Google Scholar | Crossref | Medline | |
| Nieto-Arribas, P, Seseña, S, Poveda, JM, Palop, L, Cabezas, L (2010) Genotypic and technological characterization of Leuconostoc isolates to be used as adjunct starters in Manchego cheese manufacture. Food Microbiology 27: 85–93. Google Scholar | Crossref | Medline | |
| Nieto-Arribas, P, Seseña, S, Poveda, JM, Palop, L, Cabezas, L (2011a) Genotypic and technological characterization of Lactococcus lactis isolates involved in processing of artisanal Manchego cheese. Journal of Applied Microbiology 107: 1505–1517. Google Scholar | Crossref | |
| Nieto-Arribas, P, Seseña, S, Poveda, JM, Chicón, R, Cabezas, L, Palop, L (2011b) Enterococcus populations in artisanal Manchego cheese: Biodiversity, technological and safety aspects. Food Microbiology 28: 891–899. Google Scholar | Crossref | Medline | |
| Northup, DE, Snider, JR, Spilde, MN, Porter, ML, Van de Kamp, JL, Boston, PJ(2010) Diversity of rock varnish bacterial communities from Black Canyon. Journal of Geophysical Research: Biogeosciences 115: G2. Google Scholar | |
| Parente, E, Cogan, TM (2004) Starter cultures: General aspects. In: Fox, PF, McSweeny, PLH, Cogan, TM, Guinee, TP (eds) Cheese: Chemistry, Physics and Microbiology, London: Elsevier, pp. 123–147. Google Scholar | Crossref | |
| Picon, A, Garde, S, Ávila, M, Nuñez, M (2016) Microbiota dynamics and lactic acid bacteria biodiversity in raw goat milk cheeses. International Dairy Journal 58: 14–22. Google Scholar | Crossref | |
| Ribeiro, SC, Coelho, MC, Todorov, SD, Franco, BDGM, Dapkevicius, MLE, Silva, CCG (2013) Technological properties of bacteriocin-producing lactic acid bacteria isolated from Pico cheese an artisanal cow’s milk cheese. Journal of Applied Microbiology 116: 573–585. Google Scholar | Crossref | Medline | |
| Riquelme, C, Câmara, S, Dapkevicius, MLNE, Vinuesa, P, Silva, CCG, Malcata, FX(2015) Characterization of the bacterial biodiversity in Pico cheese (an artisanal Azorean food). Journal of Applied Microbiology 192: 86–94. Google Scholar | |
| Silva, CCG, Domingos-Lopes, MFP, Magalhães, VAF, Freitas, DASR, Coelho, MC, Rosa, HJD(2015) Short communication: Latin-style fresh cheese enhances lactic acid bacteria survival but not Listeria monocytogenes resistance under in vitro simulated gastrointestinal conditions. Journal of Dairy Science 98: 1–7. Google Scholar | Crossref | Medline | |
| Siqueira, AA, Carvalho, PGS, Mendes, MLM, Shiosaki, RK (2014) MicroFit: A free software for the development and fit of mathematical models for bacterial growth. Brazilian Journal of Food Technology 17: 329–339. Google Scholar | Crossref | |
| Smitinont, T, Tansakul, C, Tanasupawat, S, Keeratipibul, S, Navarini, L, Bosco, M(2007) Exopolysaccharide-producing lactic acid bacteria strains from traditional Thai fermented foods: Isolation, identification and exopolysaccharide characterization. International Journal of Food Microbiology 51: 105–111. Google Scholar | Crossref | |
| SREA (2013) Os Açores em Números 2013, Açores: Author. Google Scholar | |
| Tagg, JR, McGiven, AR (1971) Assay system for bacteriocins. Applied Microbiology 21: 943. Google Scholar | Crossref | Medline | |
| Terzic-Vidojevic, A, Veljovic, K, Tolinacki, M, Nikolic, M, Ostojic, M, Topisirovic, L (2009) Characterization of lactic acid bacteria isolated from artisanal Zlatar cheese produced at two different geographical location. Genetika 41: 117–136. Google Scholar | Crossref |
