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First published November 2006

Interleukin-6-induced Activation of Signal Transducer and Activator of Transcription-3 in Ruptured Rotator Cuff Tendon

K Nakama, M Gotoh [email protected], T Yamada, Y Mitsui, H Yasukawa, T Imaizumi, F Higuchi, and K NagataView all authors and affiliations

https://doi.org/10.1177/147323000603400607

Abstract

The aim of this study was to examine interleukin-6 production and the activation of signal transducer and activator of transcription-3 (STAT3) in ruptured rotator cuff tendon. Specimens of ruptured rotator cuff tendons were analysed using real-time reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Specimens of co-existing inflammatory subacromial synovia were examined for comparison. The level of interleukin-6 messenger RNA was increased in ruptured rotator cuff tendon as well as in subacromial synovium. Western blot analysis showed constitutive production of activated, phosphorylated STAT3 in ruptured rotator cuff tendon and co-existing subacromial synovium. Immunohistochemical examination detected cells producing interleukin-6, interleukin-6 receptor and phosphorylated STAT3 in ruptured rotator cuff tendon, mainly in proliferative vessels and, to a lesser extent, in tendon fibroblasts around the vessels. This study demonstrates that activation of STAT3 induced by interleukin-6 is promoted mainly by proliferative vessels in ruptured rotator cuff tendon.

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Published In

Journal of International Medical Research

Volume 34, Issue 6

Pages: 624 - 631

Article first published: November 2006

Issue published: November 2006

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© 2006 SAGE Publications.

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PubMed: 17294994

Authors

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K Nakama

Department of Orthopaedic Surgery, Kurume University, Fukuoka, Japan

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M Gotoh

Department of Orthopaedic Surgery, Kurume University Medical Centre, Fukuoka, Japan

[email protected]

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T Yamada

Department of Orthopaedic Surgery, Kurume University, Fukuoka, Japan

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Y Mitsui

Department of Orthopaedic Surgery, Kurume University, Fukuoka, Japan

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H Yasukawa

Cardiovascular Research Institute and Internal Medicine III, Kurume University, Fukuoka, Japan

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T Imaizumi

Cardiovascular Research Institute and Internal Medicine III, Kurume University, Fukuoka, Japan

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F Higuchi

Department of Orthopaedic Surgery, Kurume University Medical Centre, Fukuoka, Japan

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K Nagata

Department of Orthopaedic Surgery, Kurume University, Fukuoka, Japan

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Notes

Address for correspondence Dr M Gotoh Department of Orthopaedic Surgery, Kurume University Medical Centre, 155 Kokubu-machi, Kurume, Fukuoka 839-0863, Japan. E-mail: [email protected]

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