Expression, Localization, and Significance of Vascular Permeability/Vascular Endothelial Growth Factor in Nasal Polyps
Abstract
Background
The exact etiologic mechanisms leading to the formation of nasal polyps have remained largely obscure. A key phenomenon of this specific type of chronic inflammatory disease in nasal respiratory mucosa is remarkable edema. Vascular permeability/vascular endothelial growth factor (VPF/VEGF) plays an important role in inducing angiogenesis and modulating capillary permeability.
Objective
To study the expression and localization of VPF/VEGF as a putative key factor in nasal polyp development.
Methods
Specimens of nasal polyps (n = 12) were harvested during endonasal sinus surgery in patients with polypous chronic rhinosinusitis. Specimens of healthy nasal respiratory mucosa (n = 12) served as controls and were obtained from inferior turbinates of patients undergoing surgery for nasal obstruction without signs and symptoms of inflammatory disease. Frozen sections were immunohistochemically stained for VPF/VEGF and quantitatively analyzed, using computer-based image analysis.
Results
The expression of VPF/VEGF in specimens of nasal polyps was significantly stronger than in specimens of healthy nasal mucosa of controls. VPF/VEGF in polypous tissue was mainly localized in vascular endothelial cells, in basal membranes and perivascular spaces, and in epithelial cells.
Conclusion
The markedly increased expression in nasal polyps as opposed to healthy nasal mucosa suggests that VPF/VEGF may play a significant role in both the formation of nasal polyps and in the induction of heavy tissue edema. This finding is discussed with respect to the differential expression of cyclooxygenase (COX) isoenzymes-1 and -2 (COX-1 and COX-2) in nasal polyps.
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References
1. Kaliner M.A., Osguthrope J.D., Fireman P.et al. Sinusitis: Bench to bedside. Current findings, future directions. J Allergy Clin Immunol 99: 829–848, 1997.
2. National Center of Health Statistics. Vital Health Stat 10: 199, 1995.
3. National Center of Health Statistics. Vital Health Stat 10: 190, 1994.
4. Larsen P.L., Tingsgaard P.K., Harcourt J.et al. Nasal polyps and their relation to polyps/hypertrophic polypoid mucosa in the paranasal sinuses. A macro-, endo-, and microscopic study of autopsy materials. Am J Rhinol 247: 63–76, 1998.
5. Coste A.J.G., Rateau F., Roudot-Thoraval C.et al. Increased epithelial cell proliferation in nasal polyps. Arch Otolaryngol Head Neck Surg 122: 432–436, 1996.
6. Guo J., Wang X., Tao G. Expression of vascular endothelial growth factor and transforming growth factor-beta 1 in nasal polyps. Zhonghua Er Bi Yan Hou Ke Za Zhi 36: 83–86, 2001.
7. Coste A., Brugel L., Maitre B.et al. Inflammatory cells as well as epithelial cells in nasal polyps express vascular endothelial growth factor. Eur Respir J 15: 367–372, 2000.
8. Jiang S., Dong Z., and Yang Z. Expression and the role of vascular endothelial growth factor mRNA in nasal polyps. Lin Chuang Er Bi Yan Hou Ke Za Zhi 15: 339–340, 2001.
9. Ito A., Hirota S., Mizumo H.et al. Expression of vascular permeability factor (VPF/VEGF) messenger RNA by plasma cells: Possible involvement in the development of edema in chronic inflammation. Pathol Int 45: 715–720, 1995.
10. Wittekindt C., Hess A., Bloch W.et al. Immunohistochemical expression of VEGF and VEGF receptors in nasal polyps as compared to normal turbinate mucosa. Eur Arch Otorhinolaryngol 259: 294–298, 2002.
11. Yang J., Kong H., and Dong Z. Expression and significance of vascular permeability factor in nasal polyps. Zhonghua Er Bi Yan Hou Ke Za Zhi 33: 297–298, 1998.
12. Settipane G.A. Epidemiology of nasal polyps. Allergy Asthma Proc 17: 231–236, 1996.
13. Gosepath J., Hoffmann F., Schaefer D.et al. Aspirin intolerance in patients with chronic sinusitis. ORL J Otorhinolaryngol Spec 3: 146–150, 1999.
14. Gosepath J., and Mann W.J. Aspirin intolerance and nasal polyps. Curr Opin 10: 3–7, 2002 Feb.
15. Pinto S., Gallo O., Polli G.et al. Cyclooxygenase and lipoxygenase metabolite generation in nasal polyps. Prostaglandins Leukot Essent Fatty Acids 57(6): 533–537, 1997.
16. Picado C., Fernandez-Morata J.C., Juan M.et al. Cyclooxygenase-2 mRNA is downexpressed in nasal polyps from aspirin-sensitive asthmatics. Am J Respir Crit Care Med. 160(1): 291–296, 1999.
17. Mullol J., Fernandez-Morata J.C.et al. Cyclooxygenase 1 and cyclooxygenase 2 expression is abnormally regulated in human nasal polyps. J Allergy Clin Immunol. 109(5): 824–830, 2002.
18. Gosepath J., Brieger J., Gletsou E., and Mann W.J. Expression and localization of cyclo-oxygenases (Cox-1 and Cox-2) in nasal respiratory mucosa. Does Cox-2 play a key role in the immunology of nasal polyps? J Investig Allergol Clin Immunol 14(2): 114–118, 2004.
19. Fujita T., Matsui M., Takaku K.et al. Size and invasion dependent increase in cyclooxygenase-2 levels in human colorectal carcinomas. Cancer Res 58: 4823–4826, 1998.
20. Uefuji K., Ichikura T., and Mochizuki H. Expression of cyclooxygenase-2 in human gastric adenomas and adenocarcinomas. J Surg Oncol 71: 26–30, 2001.
21. Seno H., Oshima M., Ishikawa T.O.et al. Cyclooxygenase-2 and prostaglandin E (2) receptor EP (2)-dependent angiogenesis in APC (Delta 716) mouse intestinal polyps. Cancer Res 62: 506–511, 2002.
22. Tsujii M., Kawano S., Tsuji S.et al. Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell 87: 803–809, 1996.
23. Lehr H.A., Mankoff D.A., Corwin D.et al. Application of photoshop-based image analysis to quantification of hormone receptor expression in breast cancer. J Histochem Cytochem 45: 1559–1565, 1997.
24. Lehr H.A., Jacobs T.W., Yaziji H.et al. Quantitative evaluation of HER-2/neu status in breast cancer by fluorescence in situ hybridization and by immunohistochemistry with image analysis. Am J Clin Pathol 115: 814–822, 2001.
25. Kawada M., Seno H., Wada M.et al. Cyclooxygenase-2 expression and angiogenesis in gastric hyperplastic polyp—Association with polyp size. Digestion 67: 20–24, 2003.
26. Hasegawa K., Ichikawa W., Fujita T.et al. Expression of cyclooxygenase-2 mRNA in human colorectal adenomas. Eur J Cancer 37: 1469–1474, 2001.
27. Ristimaki A., Honkanen N., Jankala H.et al. Expression of cyclooxygenase-2 in human gastric cancer. Cancer Res 57: 1276–1280, 1997.
28. Rajnakowa A., Moochhala S., Goh P.M., and Ngoi S. Expression of nitric oxide synthase, cyclooxygenase and p53 in different stage of human gastric cancer. Cancer Lett 172: 177–185, 2001.
29. Fenoglio-Preiser C.M. Gastrointestinal pathology. An Atlas and Text, ed 2. New York: Lippincott-Raven. 1998.
30. Maekawa M., Sugano K., Sano H.et al. Increased Cyclooxygenase-2 to -1 in human colorectal cancers and adenomas, but not in hyperplastic polyps. Jpn J Clin Oncol 28: 421–426, 1998.
31. Yun C.B., Lee B.H., and Jang T.J. Expression of glucocorticoid receptors and cyclooxygenase-2 in nasal polyps from nonallergic patients. Ann Otol Rhinol Laryngol. 111(1): 61–67, 2002.
32. Fernandez-Morata J.C., Mullol J., Fuentes M.et al. Regulation of cyclooxygenase-1 and -2 expression in human nasal mucosa. Effects of cytokines and dexamethasone. Clin Exp Allergy. 30(9): 1275–1284, 2000.
33. Liu C.M., Hong C.Y., Shun C.T.et al. Inducible cyclooxygenase and interleukin 6 gene expressions in nasal polyp fibroblasts: Possible implication in the pathogenesis of nasal polyposis. Arch Otolaryngol Head Neck Surg. 128(8): 945–951, 2002.
34. Sousa A., Pfister R., Christie P.E.et al. Enhanced expression of cyclo-oxygenase isoenzyme 2 (COX-2) in asthmatic airways and its cellular distribution in aspirin-sensitive asthma. Thorax. 52(11): 940–945, 1997.
35. Bruynzeel P.L., Kok P.T., Hamelink M.L.et al. Exclusive leukotriene C4 synthesis by purified human eosinophils induced by opsonized zymosan. FEBS Lett. 189(2): 350–354, 1985.
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Article first published: January/February 2005
Issue published: January/February 2005
PubMed: 15794068
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